File:Accumulative-Difference-Image-Protocol-for-Particle-Tracking-in-Fluorescence-Microscopy-Tested-in-pone.0012216.s013.ogv
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Accumulative-Difference-Image-Protocol-for-Particle-Tracking-in-Fluorescence-Microscopy-Tested-in-pone.0012216.s013.ogv (Ogg Theora video file, length 44 s, 128 × 128 pixels, 309 kbps, file size: 1.63 MB)
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[edit]DescriptionAccumulative-Difference-Image-Protocol-for-Particle-Tracking-in-Fluorescence-Microscopy-Tested-in-pone.0012216.s013.ogv |
English: The movie reports the reconstruction of the trajectory of one SLN in the cells (target 11 in Fig. 4). The images are reported in direct gray levels (all the other movies are in inverted gray levels). The trajectory lasts for 440 frames and therefore two different color rendering is given here. In this movie all the frames are inserted and the trajectory is reproduced in two different parts, both rendered in the jet(128) colormap. For the first 220 frames only the first half of the trajectory is reported on the movie. For the second 220 frames only the second half of the trajectory is reported on the movie, in the same jet(128) colormap. It is noteworthy that, despite the high density of targets in the image and the several interactions between them (20<frames<110, 180<frame<210; 320<frame<440) the trajectory is fully reconstructed with a low level of ambiguity. |
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Source | Movie S11 from Villa C, Caccia M, Sironi L, D'Alfonso L, Collini M, Rivolta I, Miserocchi G, Gorletta T, Zanoni I, Granucci F, Chirico G (2010). "Accumulative Difference Image Protocol for Particle Tracking in Fluorescence Microscopy Tested in Mouse Lymphonodes". PLOS ONE. DOI:10.1371/journal.pone.0012216. PMID 20808918. PMC: 2923183. | ||
Author | Villa C, Caccia M, Sironi L, D'Alfonso L, Collini M, Rivolta I, Miserocchi G, Gorletta T, Zanoni I, Granucci F, Chirico G | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 00:40, 28 May 2013 | 44 s, 128 × 128 (1.63 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Author | Villa C, Caccia M, Sironi L, D'Alfonso L, Collini M, Rivolta I, Miserocchi G, Gorletta T, Zanoni I, Granucci F, Chirico G |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | The movie reports the reconstruction of the trajectory of one SLN in the cells (target 11 in Fig. 4). The images are reported in direct gray levels (all the other movies are in inverted gray levels). The trajectory lasts for 440 frames and therefore two different color rendering is given here. In this movie all the frames are inserted and the trajectory is reproduced in two different parts, both rendered in the jet(128) colormap. For the first 220 frames only the first half of the trajectory is reported on the movie. For the second 220 frames only the second half of the trajectory is reported on the movie, in the same jet(128) colormap. It is noteworthy that, despite the high density of targets in the image and the several interactions between them (20<frames<110, 180<frame<210; 320<frame<440) the trajectory is fully reconstructed with a low level of ambiguity. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010 |