File:A-microRNA-initiated-DNAzyme-motor-operating-in-living-cells-ncomms14378-s2.ogv
From Wikimedia Commons, the free media repository
Jump to navigation
Jump to search
No higher resolution available.
A-microRNA-initiated-DNAzyme-motor-operating-in-living-cells-ncomms14378-s2.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 25 s, 787 × 576 pixels, 98 kbps overall, file size: 304 KB)
File information
Structured data
Captions
Summary
[edit]DescriptionA-microRNA-initiated-DNAzyme-motor-operating-in-living-cells-ncomms14378-s2.ogv |
English: Supplementary Movie 1a Imaging of fluorescence of Cy5 from individual AuNPs. Fluorescence images demonstrate operation of the DNAzyme motor on individual AuNPs in the presence of target miRNA sequence. The white dots on black background are due to fluorescent Cy5 on the AuNPs as depicted in Supplementary Fig. 14. The increases in fluorescence over time represent the miRNA-initiated stepwise walking of the DNAzyme motor on the AuNP and the corresponding catalytic cleavage of the quencher-labeled substrate. Forty five microliters of the operating solutions contained either 300 pM target miRNA, the DNAzyme motor (Supplementary Fig. 14) at an equivalent concentration of 30 pM functionalized AuNP, 25 mM Tris-acetate buffer (pH 8.0), and 200 mM NaCl. After incubation at room temperature for 20 min, 0.5 mM MnCl2 solution was added to initiate the operation of the motor. The time 0 min in the figure refers to when the MnCl2 was added. One μL of the incubation mixture was transferred to a microscope slide (25 –76 –1.0 mm; Fisher). A micro coverglass (diameter: 18 mm, thickness: 0.16-0.19 mm; Electron Microscopy Sciences) was placed over the solution on top of the slide. The AuNPs sandwiched between the microscope slide and the coverglass were then imaged using a DeltaVision OMX Imaging System (GE Healthcare Life Sciences). A 60X/1.49 TIRF objective (Nikon) was used, and a 647-nm laser provided excitation. One hundred and twenty frames were acquired in 30 min with 10% laser power and 100 ms exposure time for each frame. |
||
Date | |||
Source | Video file from Peng H, Li X, Zhang H, Le X (2017). "A microRNA-initiated DNAzyme motor operating in living cells". Nature Communications. DOI:10.1038/ncomms14378. PMID 28262725. PMC: 5343503. | ||
Author | Peng H, Li X, Zhang H, Le X | ||
Permission (Reusing this file) |
This file is licensed under the Creative Commons Attribution 4.0 International license.
|
||
Provenance InfoField |
|
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 14:42, 21 May 2017 | 25 s, 787 × 576 (304 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
Transcode status
Update transcode statusMetadata
This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Short title | Supplementary Movie 1a |
---|---|
Author | Peng H, Li X, Zhang H, Le X |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Imaging of fluorescence of Cy5 from individual AuNPs. Fluorescence images demonstrate operation of the DNAzyme motor on individual AuNPs in the presence of target miRNA sequence. The white dots on black background are due to fluorescent Cy5 on the AuNPs as depicted in Supplementary Fig. 14. The increases in fluorescence over time represent the miRNA-initiated stepwise walking of the DNAzyme motor on the AuNP and the corresponding catalytic cleavage of the quencher-labeled substrate. Forty five microliters of the operating solutions contained either 300 pM target miRNA, the DNAzyme motor (Supplementary Fig. 14) at an equivalent concentration of 30 pM functionalized AuNP, 25 mM Tris-acetate buffer (pH 8.0), and 200 mM NaCl. After incubation at room temperature for 20 min, 0.5 mM MnCl2 solution was added to initiate the operation of the motor. The time 0 min in the figure refers to when the MnCl2 was added. One μL of the incubation mixture was transferred to a microscope slide (25 –76 –1.0 mm; Fisher). A micro coverglass (diameter: 18 mm, thickness: 0.16-0.19 mm; Electron Microscopy Sciences) was placed over the solution on top of the slide. The AuNPs sandwiched between the microscope slide and the coverglass were then imaged using a DeltaVision OMX Imaging System (GE Healthcare Life Sciences). A 60X/1.49 TIRF objective (Nikon) was used, and a 647-nm laser provided excitation. One hundred and twenty frames were acquired in 30 min with 10% laser power and 100 ms exposure time for each frame. |
Software used | |
Date and time of digitizing | 2017-03-06 |